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Figure 2 | BMC Developmental Biology

Figure 2

From: Functionality of the GAL4/UAS system in Tribolium requires the use of endogenous core promoters

Figure 2

The GAL4/UAS system is active in several Tribolium stages and tissues. Animals positive for both, the GAL4Δ driver and UAS responder construct, show strong tGFP fluorescence after heat shock. Shown are pupae (a-d) and adults (e-h). (a, e) No fluorescence is visible in the negative control (heat-shocked animals that carry only the responder line UAS-Tc-bhsp-tGFP#7, i.e. eyes with red fluorescence only). (b, f) Without heat shock, animals carrying driver (Tc-hsp-GAL4Δ#1) and responder (UAS-Tc-bhsp-tGFP#7) - eyes marked red and blue - do not show body fluorescence. (c, g) Immediately after heat shock, animals carrying driver and responder construct exhibit no fluorescence of tGFP. (d, h) The same animals 24 h after heat shock exhibit strong tGFP fluorescence. Note expression in the adult wings (h). (i-u). Fluorescence is detected in some internal organs upon activation of the system (m,q,u) but not before heat shock (k, o, s; note low level of autofluorescence at different wave length). Tested were male reproductive organs (i-m), female reproductive organs (n-q) and gut (r-u). (i, l, n, p, r, t) are the respective bright field pictures.

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