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Figure 8 | BMC Developmental Biology

Figure 8

From: Emergence and migration of trunk neural crest cells in a snake, the California Kingsnake (Lampropeltis getula californiae)

Figure 8

Peripheral ganglia development in snake embryos. Wholemount staining of snake embryo with Tuj1 (green in A and B) or double stained with Tuj1 (green) and HNK1 (red) (C-I). DRG development progresses in a rostral to caudal manner: cervical DRGs are clearly mature (arrowheads in B) while those in the second coil and tail are not positive for Tuj1 (arrows in A and E). Only at more rostral levels we observe Tuj1 and HNK1 overlap (red and green arrowheads in F). G shows second coil where Tuj1 exhibited a spotted pattern, while HNK1 labeled the cells around the aorta as in other figures (arrowheads). H shows a higher magnification of G. I show a higher magnification of first coil. Blue stain corresponds to DAPI labeled nuclei. R is for rostral; C is for caudal orientation in the embryo coils.

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