Figure 7
DiI and HNK1 labels neural crest cells. DiI (red) and HNK1 (green) st.21 stained snake embryo No.5 sections labels a variety of migrating neural crest. A-C show wholemount at the level of second coil, which label DiI migrating cells co-localizing with HNK1 around aorta/ventral portion of trunk (white arrows). There were many DiI cells around the mesonephroi (red arrows). D-F show a section on the caudal portion of 1st coil. The only DiI cells were localized in the DRG, which also was strongly stained with HNK1. Other cells were only DiI (red arrows) or only HNK1 (white arrows). G-I shows a longitudinal section on the second coil where DiI and HNK1 co-localized, especially in the condensing DRG. Not all DiI were HNK1 positive and vice-versa. J shows a higher magnification of one longitudinal section of DiI positive neural crest migrating between the somites (arrows) while HNK1 cells are found in the rostral portion of the somite. K corresponds to a section on the 2nd coil showing DiI co-localizing with HNK1 around the mesonephroi/kidneys (re.Fig. 5G-I). L is a cartoon depicting the axial levels of the sections shown in this figure. R is for rostral; C is for caudal orientation in the embryo coils.