Figure 5
Wholemount immuno-staining with HNK1. Snake embryo at st.21 (4POV) was wholemounted with HNK1 antibody (red) and DAPI (blue). A and B show head and trunk coils respectively. C and D show sensory ganglia positive for HNK1 at the vagal level. Arrows point spinal nerves positive for HNK1. E and F show HNK1 cells migrating along ventromedial pathway (arrows in E) at tail portion and crest cells at the sympathetic/aorta level or mesonephroi (arrowheads in F) at the midtrunk portion. G corresponds to the tail-most end, where the first neural crest cells are migrating (arrows). H shows the tail end providing a lower magnification of E and G areas. MN: mesonephroi. Symp: sympathetic. R corresponds to rostral and C to caudal.