Figure 2

Structure of the DelTel7 allele and absence of Kcnq1ot1 in rescued DelTel7/IC2KO embryos. A) Simplified representation of the structure of distal Chr7 in the DelTel7/IC2KO rescued animals, revealing the DelTel7 truncation on the maternal allele and the absence of IC2 and lack of activation of Kcnq1ot1 expression on the paternal allele. M = maternal; P = paternal. C) RT-PCR demonstrating lack of Kcnq1ot1 expression in E9.5 DelTel7/IC2KO embryo and placenta.