Figure 8

Cell sorting analysis demonstrates the presence of putative NSCs resident in the in vitro NSC niche. A, Gates for "live" cells included dot plot of side scatter (ssc) versus GFP fluorescence (FL1-GFP). The cellular population was fairly uniform in scatter on Days 0-8, but showed greater variety in Days 14-20 as the GFP⁺ cells adopt their mature cell-fates. B, There is an increase in the number of CD133 labeled cells on Day 20 when compared to Day 0. C, Cells gated through the live gate in (A) and the CD133⁺ gate in (B) are further analyzed for PDGFRα and GFAP expression. The upper right quadrant indicates cells that are positive for all three markers. D, Percentages of cells on different days of culture that express CD133, PDGFRα and GFAP, or all three markers. Day 0 represents undifferentiated mouse embryonic stem cells in culture; Days 4 and 8 are taken from cells during the 4-/4+ neural induction; Days 14, 16 and 20 are taken from cells in the in vitro NSC niche after the 4-/4+ neural induction.