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Figure 6 | BMC Developmental Biology

Figure 6

From: Developmental cues and persistent neurogenic potential within an in vitro neural niche

Figure 6

Quantitative RT-PCR of genes indicative of particular developmental lineages. A, C and E, Fold changes in expression levels of (A) NSC-associated genes, (C) mature neural cell-associated genes, (E) mesodermal and endodermal-associated genes in the developing in vitro NSC niche compared to Day 0 mouse embryonic stem cell cultures. B, D and F, Fold changes of (B) NSC-associated genes, (D) mature neural cell-associated genes, (F) mesodermal and endodermal-associated genes in the developing in vitro NSC niche compared to cells that have undergone 4-/4+ retinoic acid induction (Day 8). Of the genes tested (see methods) only those genes with statistically significant (p < 0.05) differences from Day 8 cultures are shown. TATA box binding protein (TBP) is used as the housekeeping gene in all experiments. Data supporting our housekeeping gene selection are provided in Additional file 6. Genes: Paired box gene 6 (Pax6), Platelet derived growth factor receptor alpha (PDGFRα), Glial fibrillary acidic protein (GFAP), Distal-less homeobox 2 (Dlx2), Prominin 1 (Prom1, also CD133), Chondroitin sulfate proteoglycan 4 (Cspg4), Microtubule-associated protein 2 (Map2a), β-III tubulin, Brachyury, Platelet/endothelial cell adhesion molecule 1 (Pecam1), GATA binding protein 4 (Gata4), Alpha fetoprotein (Afp) and TATA box binding protein (TBP).

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