Figure 2
From: Developmental cues and persistent neurogenic potential within an in vitro neural niche
RT-PCR profile of in vitro NSC niche by day of induction. Day 0 represents undifferentiated mouse embryonic stem cells. Day 4 and Day 8 represent suspended embryoid bodies during 4-/4+ retinoic acid neural induction; Day 4 cells are harvested prior to the addition of retinoic acid, and Day 8 is harvested after 4 days of retinoic acid exposure. Days 10, 12 and 14 represent time-points of further neural induction as attached cultures on ECL. A, Neural markers during culture development. B, Retinoic acid receptors and caspase-3 through Day 14. (Days 12 and 14 and their housekeeping gene run together but on a second gel) C, Extracellular matrix (ECM) components and receptors during culture development.