Figure 3

Overexpression of RNCR2 in the developing retina. CAG-GFP or CAG-RNCR2 construct was electroporated into P0.5 retina and harvested at P21, dissociated and stained for cell-specific markers. GFP-positive cells were counted to analyze the fraction of electroporated cells that expressed the markers in question. Error bars represent +/- SEM for at least three independent retinas. (A) CAG-RNCR2 overexpression has no effect on cell fate determination. The number of GFP-positive electroporated cells in ONL and INL were counted and compared (inset), with no difference was noted. (B-C) Section immunohistochemistry of retinas electroporated with either CAG-GFP or CAG-RNCR2 and harvested at P21 confirm no differences in cell fate or morphology of CAG-RNCR2 electroporated cells. Cell type specific markers used: rhodopsin (Rho4D2), rod photoreceptors, glutamine synthetase (GS), Müller glia; protein kinase C alpha (PKCα), rod bipolar cells; syntaxin (Syn), amacrine cells.