Figure 8
From: Conditional expression of Spry1 in neural crest causes craniofacial and cardiac defects
Impaired cranial nerve development in Spry1;Wnt1-Cre embryos. (A) Whole mount staining with neurofilament antibody at E10.5 shows smaller nerve filament bundles in Spry1Wnt1-Cre (Spry1) embyos compared to Cre-negative littermate controls (WT). (B) Higher magnification to show the defect in cranial nerves IX and X in Spry1;Wnt1-Cre transgenic embryos (* indicates misplaced cranial nerves). (C, D) Hematoxylin and eosin staining of E16.5 cross sections to show the smaller dorsal root ganglia in Spry1;Wnt1-Cre embryos compared to littermate controls (arrow indicated). (D) High magnification from boxed areas in C. IX: glossopharyngeal nerve; X: vagus nerve. Six Spry1;Wnt1-Cre and six control embryos representing two litters were analyzed.