Figure 6
From: Conditional expression of Spry1 in neural crest causes craniofacial and cardiac defects
Overexpression of Spry1 in neural crest cells decreased expression of craniofacial marker genes at E10.5. (A, B) Overexpression of Spry1 decreases the expression domains of AP2α, Msx1 and Msx2 expression. (A) Lateral view to show the decreased expression of AP2α, Msx1 and Msx2 in the branchial arches of Spry1;Wnt1-Cre (Spry1) embryos, (B) frontal view to show the decreased expression of AP2α, Msx1 and Msx2 in maxilla and nasal pits of Spry1;Wnt1-Cre embryos. (C, D) Overexpression of Spry1 reduced expression domains of Dlx5 and Dlx6. (C) Lateral view to show the decreased expression of Dlx5 and Dlx6 in branchial arches of Spry1;Wnt1-Cre embryos. (D) Frontal view to show the decreased expression domains of Dlx5 and Dlx6 in maxilla and nasal pits of Spry1;Wnt1-Cre embryos compared to control (WT) mice. All in situ hybridization experiments were performed at least three times on Spry1;Wnt1-Cre embryos and their Cre-negative littermates. Color development time was equivalent for each experiment.