Figure 5
From: Conditional expression of Spry1 in neural crest causes craniofacial and cardiac defects
Overexpression of Spry1 in neural crest-derived cells induced apoptosis and reduced cell proliferation. (A-D) Phospho-histone H3 immunofluorescence staining of E10.5 embryo cross sections showed the reduced phospho-histone H3 positive cells in Spry1;Wnt-Cre (Spry1) embryos compared to Cre-negative littermate controls(WT) (from original 40×). Low magnification to show similarity of sections (from original 10×) (A), high magnification to show the decreased phospho-histone H3 positive cells in neural tube (B) and branchial arches (C) of Spry1;Wnt1-Cre mice compared to WT control (from original 40×). (D) Quantification of phospho-histone H3 positive cells. (E-G) TUNEL labeling shows an increase in apoptotic cells in the neural tube of Spry1;Wnt1-Cre embryos but not in control embryos (from original 40×). (H) Quantification of TUNEL labeled cells. NT: neural tube; BA: branchial arch. Data shown are representative of three independent experiments.