Figure 4
From: Conditional expression of Spry1 in neural crest causes craniofacial and cardiac defects
Spry1;Wnt1-Cre embryos have reduced β-gal staining in neural crest-derived structures. (A-D) Whole mount β-gal staining, (A, B) lateral view to show neural crest cells (blue) have contributed to the branchial arches and lateral mesoderm both in Spry1;Wnt1-Cre (Spry1) and WT control mice, however the β-gal stained neural crest derivatives in Spry1 embryos were smaller than those of the controls (WT) (arrows indicated). (C, D) Dorsal view to show the similar intensity of β-gal staining at the ridge of the neural tube of R26R;Wnt1-Cre (WT) or Spry1;R26R;Wnt1-Cre embryos. (E-H) Cross sections of E9.5 whole mount β-gal stained embryos. (E, F) Low magnification to show intensity of β-gal staining in ridge of neural tube and brachial arches of WT and Spry1;Wnt1-Cre embryos. (G, H) High magnification to show the decreased cellular mass in the first branchial arch of Spry1;Wnt1-Cre embryos with reduced β-gal staining. Data are representative of three independent experiments.