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Figure 2 | BMC Developmental Biology

Figure 2

From: Conditional expression of Spry1 in neural crest causes craniofacial and cardiac defects

Figure 2

Spry1;Wnt1-Cre embryos exhibit craniofacial defects. (A-C) E16.5 embryo imagines, (A) Lateral view of whole embryos (from original 1×), (B) frontal and (C) lateral views of head (from original 2.5 ×) shows facial clefting in Spry1;Wnt1-Cre (Spry1) embryos. (D-H) Skeletal preparations of E16.5 embryos stained by alcian blue and alizarin red, (D) lateral and (E) basal views, the mandibles were removed to enhance the view of the of cranial base, arrows indicate absent bones or abnormal elements. (F) The mandible was shorter in Spry1;Wnt1-Cre embryos compared to WT controls. (G-H) Skeletal preparations of newborn mice, (G) dorsal and (H) basal views. Data presented are representative of five litters analyzed at this gestational age. Na: nasal capsule; nb: nasal bone; fn: front bone; bs: basisphenoid; eo: exooccipital; ip: interparietal bone; mx maxilla; md: mandible; ob: basioccipital; pa: parietal bone; ps: presphenoid; so: supraoccipital. (I) In utero MR images of WT and Spry1;Wnt1-Cre embryos at 14 dpc. The overview image shows 4 embryos in varying orientations and views. The enlarged view on the right side show (top) a WT embryo with normal facial and cardiac development and (bottom) a Spry1;Wnt1-Cre embryo with severe facial malformations, enlarged heart and defective cardiac outflow tract. Images were obtained with a RARE pulse sequence (TE 39.8 ms, TR 2571 ms, FOV 35 × 35 mm, matrix 256 × 256, slice thickness 1 mm (total of 7 slices), 3 averages, total scan time 4 min. 6 sec).

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