Figure 5

C/EBPα was required for and stimulated adipocyte differentiation from hBMSCs. (A) Relative expression levels of C/EBPα were determined at the indicated days by real-time PCR. (B) Adipocyte differentiation revealed by oil red O staining with C/EBPα knock-down by adenovirus expressing shRNA. (C) C/EBPα knock-down was confirmed by real-time PCR (n = 3, *P < 0.05). (D) C/EBPα over-expression in hBMSCs using adenovirus (Lac Z as control) was shown by Western Blotting. (E) HBMSCs were cultured to confluence and infected with adenovirus at MOI 10 followed by various combinations of hormone treatment 4 h later for three days. Cells were stained with oil red O on day eight (magnification 100×). (F) The expression of the adipocyte marker (FABP4) was detected on day four by Western Blotting.