C/EBPβ was required for and stimulated adipocyte differentiation from hBMSCs. (A) Relative expression levels of C/EBPβ were determined at the indicated days by real-time PCR. (B) Adipogeic differentiation revealed by oil red O staining with C/EBPβ knock-down by SiRNA. (C) Expression levels of C/EBPβ were determined by real-time PCR (n = 3, *P < 0.05). (D) Over-expression of C/EBPβ in hBMSCs with adenoviral infection (Lac Z as control) was confirmed by Western Blotting. (E) HBMSCs were cultured to confluence and infected with adenovirus at MOI 10 followed by various combination of hormone treatment 4 h later for three days. Cells were stained with oil red O on day eight (magnification 100×). (F) The expression of the adipocyte marker (FABP4) was detected on day four by Western Blotting.