Figure 1From: Characterization of adipocyte differentiation from human mesenchymal stem cells in bone marrowIsolation and adipogenic differentiation of hBMSCs. (A) The morphology of adherent hBMSCs three and 12 days after plating (magnification 100×). (B) HBMSCs of P5 were cultured for one week after confluence and induced to differentiate with MDI+Indo (M: methyl-isobutyl-xanthine; D: dexamethasone; I: insulin; Indo: indomethacin) treatment for one, two or three cycles (1 cycle of treatment: MDI+Indo for three days followed by insulin for one day). The accumulation of cytoplasmic triglyceride was detected by Oil Red O staining on day 21 and visualized under a microscope (magnification 100×). (C) FABP4 expression was examined by Western Blotting at the indicated days after differentiation with repeated MDI+Indo treatment (three times).Back to article page