GLI3 specific, spatiotemporal pattern of reporter gene expression in transgenic mouse embryos is evoked by intronic conserved non-coding sequence elements (CNEs) acting in a complementary fashion. (A) Human chromosome 7 coordinates along with the graphical representation of exons (numbered) and introns of GLI3. (B) Graphical plot depicting evolutionary conservation of human GLI3 across multiple mammalian vertebrates (blue) and Fugu (green) generated using sequence alignment tools in the UCSC comparative genomics alignment pipeline. http://genome.ucsc.edu. (C) 1-12: Human/Fugu CNEs characterized as enhancers through functional assays by employing human cell lines and zebrafish embryos [26, 28]. A, R: activating or repressory potential of these enhancers in cell culture. (D) Subset of 6 intronic CNEs whose spatiotemporal regulatory potential is defined in transgenic mouse assay. Selected embryos are shown at representative time points (E11.5 or E12.5) along with their primary target sites.