Figure 3

Expression of genes in the EVL. Whole mount in situ hybridizations for mRNAs expressed in the EVL encoding one RhoGEF (zgc:136817 [58]), one protein with a LIM zinc binding domain (zgc:152778 [58]), one solute carrier (slc14a2 [59]), one protein related to a family of long chain fatty acid-elongating enzymes (elovl6l [58]), one protein with a DEP domain (zgc:136359 [31]), indicating it might be involved in G-protein coupled receptor recognition, and one intracellular chloride channel protein (clica [31]). Expression of three of these genes was excluded from the margin at the sphere stage of development (D, G and J), also seen in histological sections (E, H and K). By shield stage, expression is apparent throughout the EVL (F, I and L). Lateral views are shown throughout and developmental stages and genes probed are indicated. Asterisks on the bottom right corner indicate genes for which no in situ expression data has previously been reported.