Figure 1

Flow diagram for volume rendering from serial sections. Flow diagram showing volume rendering protocols for grayscale (black pathway) and colored (red pathway) image stacks using the 3D software package Amira 4.1. Since the presented grayscale image stack of Cristatella mucedo is based on resin sections, image filtering is not explicitly necessary and VR can be performed directly on the aligned image stack. The presented colored image stack of Phoronis australis is based on paraffin wax sections, thus the pre-rendering procedure is more complex, including the application of two different 3D image filters on the single RGB channels of the stack.