Figure 6

Localization of Fry and Trc in da neurons. Panels A1-A3 show a da neuron from a ppkGAL4-UAS-mCD8RFP/UAS-fry-GFP white prepupae. A1 shows cytoplasmic RFP, A2 Fry-GFP and A3 a merged image. Note that the GFP signal is excluded from nucleus, but is found throughout the cytoplasm. Panels B1-3 show a da neuron from a ppkGAL4/UAS-fry-GFP;UAS-RedStinger/+ white prepupae. B1 shows nuclear RFP, B2 Fry-GFP and B3 a merged image. Note that there is little overlap between the nuclear RedStinger and Fry-GFP. The modest GFP signal over the nuclear region is sometimes seen but it is not clear if this is due to overlaying cytoplasm or actual nuclear Fry. Panels C1-3 show a da neuron from a ppkGAL4-UAS-mCD8RFP/UAS-trc-GFP white prepupae. C1 shows cytoplasmic RFP, C2 Trc-GFP and C3 a merged image. Note that the GFP signal is brighter in the nucleus than cytoplasm. Panels D1-3 show a da neuron from a ppkGAL4/UAS-trc-GFP;UAS-RedStinger/+ white prepupae. In this case the RedStinger is nuclear and once again Trc-GFP is found in both the nucleus and cytoplasm.