Figure 3

Intracellular localization of Fry in bristles and hairs. (A1) A wt bristle stained with anti-Fry antibody shows punctate localization and accumulation at the tip (arrowhead). (A2) A second bristle and a blow up (A3). (B1) Thoracic microchaetae from a living UAS-Myc-fry-GFP/+; neur-gal4/+ pupae. Note the tip accumulation of Myc-Fry-GFP (arrowheads). B2 higher magnification of B1 (C) C1 is from a pupal head stained with anti-acyl-tubulin and anti-Fry. Endogenous Fry accumulates at the tip. Blow ups of bristle tips are shown in C2-5. Similar results were obtained using anti-tubulin antibodies. (D) Growing wing hairs from a UAS-Myc-N-fry/+; actinGAL4/+ pupal wing immunostained with both anti-Myc and anti-C terminal Fry antibodies. Both types of Fry protein show a punctate distribution but the two only partly co-localize (arrowheads). Most puncta contain only one protein (arrows). (E) The distal half of a bristle from a UAS-Myc-N-fry/+; actinGAL4/+ pupa immunostained with both anti-Myc and anti-C terminal Fry antibodies. Note the distal accumulation of endogenous Fry but not Myc-N-Fry. (F) A growing wing hair from a UAS-Myc-N-fry/+; actinGAL4/+ pupal wing immunostained with both anti-Trc and anti-Myc. Note the extensive co-localization between Trc and Myc-N-Fry. Arrowheads point to two puncta that stain for both. (G) A growing bristle from a UAS-Myc-N-fry/+; actinGAL4/+ pupa immunostained with both anti-Trc and anti-Myc. Note the extensive co-localization. Arrowheads point to three puncta that stain for both. (H) A bristle from a wt pupa stained with both anti-Trc and anti-Fry antibodies. Note that only a few puncta stain with both antibodies (arrowheads).