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Table 2 Quantification of potential Muc1 contribution to endocrine cells

From: Exocrine-to-endocrine differentiation is detectable only prior to birth in the uninjured mouse pancreas

      number of cells scored
Experiment Genotype Pulse protocol Chase period Sample # lineage+ INS+ GLU+
1 Muc1 IC2/+ ; Rosa26 EYFP/+ adult (P60)
10 mg TM
gavage
60 d 1 2950 2054 ND
     2 959 1398  
     total 3909 3452  
    120 d 1 1588 624 209
     2 1416 498 144
     3 1266 1069 374
     4 2089 2004 703
     5 1199 1488 409
     6 1079 904 369
     total 8637 6587 2208
2 Muc1 IC2/+ ; Rosa26 LacZ/+ pups
(P0/P1)
10 mg TM
maternal gavage
21 d 1 221 590 276
     2 483 745 235
     3 266 1045 436
     total 970 2380 947
    120 d 1 2072 2152 626
     2 2637 1847 315
     total 4709 3999 941
3 Muc1 IC2/+ ; Rosa26 EYFP/+ pups (P0)
2 mg TM
SQ
21 d 1 1566 2017 999
     2 1264 950 488
     3 3508 1570 564
     Total 6338 4537 2051
  1. As described in the text, mice subjected to "pulse-chase" labeling with Muc1IC2 were analyzed for potential contribution of lineage-marked cells to insulin+ β-cells or glucagon+ α-cells (exocrine labeling results in Table 1). Indicated are the total number of cells scored as positive for lineage label (EYFP or LacZ), as well as the total number of insulin+ and glucagon+ cells scored in the same fields. In no case did we observe a lineage label-positive endocrine cell. ND, not determined.