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Table 2 Quantification of potential Muc1 contribution to endocrine cells

From: Exocrine-to-endocrine differentiation is detectable only prior to birth in the uninjured mouse pancreas

     

number of cells scored

Experiment

Genotype

Pulse protocol

Chase period

Sample #

lineage+

INS+

GLU+

1

Muc1 IC2/+ ; Rosa26 EYFP/+

adult (P60)

10 mg TM

gavage

60 d

1

2950

2054

ND

    

2

959

1398

 
    

total

3909

3452

 
   

120 d

1

1588

624

209

    

2

1416

498

144

    

3

1266

1069

374

    

4

2089

2004

703

    

5

1199

1488

409

    

6

1079

904

369

    

total

8637

6587

2208

2

Muc1 IC2/+ ; Rosa26 LacZ/+

pups

(P0/P1)

10 mg TM

maternal gavage

21 d

1

221

590

276

    

2

483

745

235

    

3

266

1045

436

    

total

970

2380

947

   

120 d

1

2072

2152

626

    

2

2637

1847

315

    

total

4709

3999

941

3

Muc1 IC2/+ ; Rosa26 EYFP/+

pups (P0)

2 mg TM

SQ

21 d

1

1566

2017

999

    

2

1264

950

488

    

3

3508

1570

564

    

Total

6338

4537

2051

  1. As described in the text, mice subjected to "pulse-chase" labeling with Muc1IC2 were analyzed for potential contribution of lineage-marked cells to insulin+ β-cells or glucagon+ α-cells (exocrine labeling results in Table 1). Indicated are the total number of cells scored as positive for lineage label (EYFP or LacZ), as well as the total number of insulin+ and glucagon+ cells scored in the same fields. In no case did we observe a lineage label-positive endocrine cell. ND, not determined.