The endocytic recycling regulator EHD1 is essential for spermatogenesis and male fertility in mice

Table 2 Uncorrected organ weights and seminiferous tubule widths of WT and Ehd1^-/- male mice

Age	Genotype	Testes, mg	Seminiferous Tubule Width, mm × 10^-1	Spleen, mg	Kidneys, mg
Day 10	WT	8.9 ± 1.6	1.3 ± 0.1	21.8 ± 2.2	57.6 ± 9.3
Day 10	*Ehd1* ^-/-	7.0 ± 1.0	1.2 ± 0.1	23.2 ± 8.5	46.5 ± 13.5
Day 30	WT	98.2 ± 10.7	2.9 ± 0.3	78.9 ± 13.9	238.2 ± 16.4
Day 30	*Ehd1* ^-/-	90.5 ± 8.1	3.1 ± 0.2	90.0 ± 8.0	242.2 ± 39.7
Day 42	WT	172.7 ± 5.9*	3.6 ± 0.4	83.1 ± 10.2	358 ± 43.5
Day 42	*Ehd1* ^-/-	127.7 ± 24.8*	3.6 ± 0.4	75.1 ± 6.7	324.7 ± 3.2
Weeks 9-11	WT	197.0 ± 17.5**	n/a	63.7 ± 10.6	392.7 ± 30.6
Weeks 9-11	*Ehd1* ^-/-	106.3 ± 17.1**	n/a	69.0 ± 13.1	362 ± 15.9
Weeks 19-21	WT	227.0 ± 2.0**	n/a	81.2 ± 7.0	442.4 ± 78.2
Weeks 19-21	*Ehd1* ^-/-	136.7 ± 14.4**	n/a	65.3 ± 11.6	341.3 ± 35.9
Weeks 61-69	WT	185.4 ± 31.3*	n/a	70.2 ± 7.6	502.2 ± 38.2
Weeks 61-69	*Ehd1* ^-/-	93.5 ± 6.9*	n/a	60.2 ± 11.5	468.7 ± 136.7

Organs were dissected from euthanized mice and weighed. The testes weight represents both testis dissected from the scrotal sac, the seminiferous tubule widths (n ≥ 20) were measured using ImageJ software http://rsbweb.nih.gov/ij/ to measure the smallest width of circular seminiferous tubules across the lumen from light microscopy photographs of PAS-stained Bouin's-fixed testis sections. The kidney weight represents both kidneys (n > 3 for each measurement). The uncorrected seminal vesicle weights of 61-69 week old mice: WT = 569.9 ± 184.5 mg and Ehd1^-/- = 411.1 ± 89.3 mg (p > 0.05). Statistical analysis comparing the raw data of each age-matched values between WT and Ehd1^-/- mice was determined using a two-sample t-test (* indicates p < 0.05 using one-tailed analysis, ** indicates p < 0.05 using two-tailed analysis).

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