Figure 8
From: The endocytic recycling regulator EHD1 is essential for spermatogenesis and male fertility in mice
Ehd1-/- male mice lack mature epididymal spermatozoa at day 42. Day 42 caput epididymides were Bouin's-fixed, PAS-stained and hematoxylin-counter-stained to visualize the glycoproteins (pink) and nuclei (blue) and analyzed by light microscopy using a 40× objective lens. (A) WT (+/+) epididymides contained a columnar epithelial layer (E) with a smooth actin layer (arrow) beneath the long PAS-positive microvilli that extend into the lumen. Mature spermatozoa (S) were present in the lumen. (B) The Ehd1-/- (-/-) epididymides contained a few sloughed round spermatids (Spt) and spermatocytes (Spc). Scale bar = 20 μm.