Figure 4

Ehd1 mRNA expression in post-natal mouse testis development. In situ hybridizations were performed as described in Methods on WT (+/+) and Ehd1^-/- (-/-) testis sections prepared from post-natal day 10, 30, 36, 42 (P10-P42) and 10 month old (10 m) mice. Ehd1 mRNA expression can be seen as red in dark-field images overlaid on bright-field images. Panels A'-I' are higher magnifications of panels A-I, respectively. The inset within E' is an enlarged micrograph of the box in E'; arrows denote Sertoli cell nuclei. The scale bar in panel I is 100 μm for B-J, 50 μm for A, C'-I' and 25 μm for A'.