Figure 1

Generation of Ehd1^-/- mice using Cre/ loxP -mediated genetic recombineering. (A) A partial restriction map of the Ehd1 locus, the targeting vector and the mutated Ehd1 loci. The first exon was deleted by Cre/loxP-mediated recombination. Black rectangles represent exons, grey and white triangles represent loxP and FRT sequences, respectively. H, HindIII; RI, EcoRI. (B) DNA was prepared from mouse tails for genotyping by PCR to amplify the WT Ehd1 allele, the deleted allele and/or the floxed allele. The lane labeled "no template" indicates a negative control in the absence of DNA. (C) RT-PCR analysis was carried out using cDNA generated from mouse testes and primers specific for Ehd1 and Ehd4. The primers are described in Methods.