Figure 6
Notch112f/tm1Conembryos survive longer than Notch112f/lbdembryos. (A) PCR genotype of an E9.5 litter showed the 280 bp PCR product from Notch1tm1Conallele and the 238 bp product from the Notch112fallele. (B) Yolk sac vascularization of E10.5 Notch112f/tm1Conand Notch1+/12fembryos. (C) Notch112f/tm1Conembryos at E10.5 exhibit heamorrhaging around the heart (arrows). (D) Notch112f//tm1Conand control embryos at E15.5. One Notch112f/tm1Conembryo was defective but the other had no obvious defects. (E) Notch112f/tm1Conand control embryos at E17.5. One Notch112f/tm1Conembryo was defective but the other had no obvious defects. (F) Photo of a litter on postnatal day 1 (P1) which included one pup identified as Notch112f/tm1Conby PCR genotyping below. The pup was indistingishable but died within a few days. (G) PCR genotype of the P1 litter in panel F.