Figure 5
Real-time PCR of vasculogenic and Notch target genes in Notch112f/lbdyolk sac and embryo. Total RNA extracted from E10.5 yolk sac or embryonic head was reverse-transcibed and subjected to real-time PCR. Numbers of transcripts were normalized to β-actin, and the average relative expression of Notch1+/12fsamples was set to 1. (A-F) Relative expression of Pecam1, Vegf, Vegfr2, Hes5, Hey1, and Hey2 as marked. Bars represent SEM (n = 6). The two-tailed Student's t test was used in control versus mutant yolk sac and embryo head comparisons; a one-tailed Student's t test was used in mutant yolk sac versus mutant embryo head comparisons; * p < 0.05; ** p < 0.01