Figure 4

Constructs and representative images for module A. I. Different constructs used to study the function of six3a module A. The names of those constructs were given by the length of the DNA fragment left after 5' or 3' deletion. The number after A indicates the length of module A left after 5' deletion. The small triangle indicates the length of module A left after 3' deletion. Followed by each construct are the summary of the GFP expression level at 24 hpf for different regions (eyes or forebrain) as plus sign (+) to indicate different levels of GFP. The minus symbol (-) indicates the absence of GFP expression, and plus/minus (+/-) indicates slightly higher GFP expression than no expression. II. The percentages of embryos expressing GFP from different batches are shown with the total numbers of embryos from 24 hpf (red). III. Representative GFP expression patterns for each construct in lateral views at 24 hpf. (A) A462-GFP. (B) A433-Bp-GFP. (C) A415-Bp-GFP. (D) A392-Bp-GFP. (E) A462(triangle)298-Bp-GFP (F) A433(triangle)298-Bp-GFP. (G) A462(triangle)298-Bp-M-GFP. (H) A392(triangle)298-Bp-GFP. The last panel shows the sequence in module A. Conserved sequences in module A were aligned among zebrafish, X. tropicalis, opossum, Tetraodon, human and mouse. The primers used to obtain serial deletion constructs are shown in red or green arrow with direction indicated forward or reverse. The 22-bp region (underlines with double red arrow) was deleted in A462(triangle)298-BP-M-GFP. The red boxes represent the highly conserved elements.