Figure 1

Zebrafish six3a conserved non-coding modules and map. (A) The 21-kb genomic region from -12 kb to +9373 bp, relative to the zebrafish six3a transcriptional start site. The horizontal light blue blocks at the top of the figure represent two exons of zebrafish six3a, the transcriptional start site indicated as a black arrow. Green histograms indicate the sequence similarities of different species in the corresponding regions. The conserved regions are shown as dark blue histograms under the six3a transcript. (B) Schematic structure of zebrafish six3a cis-regulatory elements. The thick black line represents the genomic sequence from -12 kb to +9937 bp. The horizontal red blocks at the top of the figure represent two exons of zebrafish six3a, and "+1" represents the transcriptional start site indicated as a black arrow. The translational initiation site is indicated as a red arrow. Conserved regions in Fig. 1A are shown by light blue blocks named, from 5' to 3': 1, 2, 3, A, B, C, D, 4, 5 and 6. Bp represents the basal promoter, shown by an orange block. Modules 1, A and 5 are conserved among all species, modules 2 and 3 show minor conservation and the remaining modules have low sequence similarity among species. The 4.5-kb upstream sequence from medaka olsix3.2 was used as comparison.