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Figure 3 | BMC Developmental Biology

Figure 3

From: The function and regulation of the bHLH gene, cato, in Drosophila neurogenesis

Figure 3

Cato has separate Ch and ES/md enhancers. (A) Schematic showing the upstream region of cato, the fragments tested by GFP reporter gene analysis, and a summary of the expression patterns supported (Ch or ES/md). The positions of the two E boxes mentioned in the text are shown, as are their sequences in D. melanogaster and pseudoobscura compared to the known Ato consensus binding site. (B-H) GFP expression in embryos containing different cato reporter genes (green). (B)cato1.6k-GFP in early stage embryo showing early expression in Ch and dbd cells. (C) cato1.6k-GFP in late stage embryo showing expression in all Ch lineages (arrows) and da neurons (costained with 22C10 in magenta). (D) cato1.6k2 M-GFP in stage 12 embryo showing expression just beginning in some ES cells but loss of expression in Ch lineages. (E) cato1.6k2 M-GFP in late stage embryo showing expression in da neurons but loss of expression in Ch lineages (arrows). (F) cato2A-GFP in early embryo showing early expression in Ch precursors (G) cato2A2 M-GFP expression in early embryo, showing lack of Ch expression. (H) cato2B-GFP in late embryo showing expression non-Ch lineages but not in Ch lineages (arrows). (I) Summary of expression pattern and reporter gene evidence that suggests that cato is a direct target of Ato (and Amos), but an indirect target of Sc.

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