Figure 2

Cato protein expression relative to late neural markers. Immunohistochemistry with anti-Cato (green) and a second marker antibody (magenta). (A) Stage 14 embryo showing substantial co-expression of Cato and Elav, a neuronal differentiation marker. (B) Larger magnification view of similar embryo showing dorsolateral regions of several abdominal segments. (C) Stage 13 embryo, showing that Cato expression generally persists longer than Ase in most cells. (D) Ventrolateral view of stage 14 embryo, showing Cato expression generally more persistent that Sens. (E-G) Expression of the cato1.6k-GFP reporter gene. (E, E') Stage 16 embryo, dorsolateral abdominal segments, showing GFP expression (green) relative to Cpo, which marks nuclei of all PNS cells (blue) and 22C10, which marks sensory neurons (red). (F) Ventrolateral view of a similar embryo. (G) Stage 16 embryo showing expression of GFP relative to Elav, ventrolateral view of abdominal segments. (H) Schematic representation of sensory neurons (filled) and support cells (unfilled) of an abdominal segment (based on [44]): Neurons are: Ch (green), ES (blue); md (red). Abbreviations: v = ventral; v' = ventral'; l = lateral; d = dorsal; ch = chordotonal; es = external sensory; da = dendritic arborisation neuron; bd = bipolar dendritic neuron; td = tracheal dendritic neuron; md = multidendritic neuron; n = neuron; sc = scolopale cell; lg = ligament cell; ca = -cap cell; g = glial cell.