Figure 3

In situ hybridization of chicken wing buds with probes against chondrogenic markers Sox9 (A, B) and Coll2a (C, D), after injection of Wnt6-CHO cells at HH-stage 20-21 and 24 or 48 h reincubation. On the left side is the control limb, the right limb has been manipulated. Dotted line indicates the site of injection. A Expression of Sox9 after 24 h. Expression is severely reduced on the operated side. a Transverse section of the control limb showing normal Sox9 expression. a' Transverse section of the operated limb showing weak Sox9 expression. B Expression of Sox9 after 48 h. Expression is reduced on the operated side. b Transverse section of the control limb showing normal Sox9 expression. b' Transverse section of the operated limb showing reduced Sox9 expression. C Expression of ColIIA after 24 h. Expression is locally reduced on the operated side. c Transverse section of the control limb showing normal ColIIA expression. c' Transverse section of the operated limb showing weak ColIIA expression. D Expression of ColIIA after 48 h. Expression is reduced on the operated side. d Transverse section of the control limb showing normal ColIIA expression. d' Transverse section of the operated limb showing reduced ColIIA expression. Scale bar 500 μm.