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Figure 2 | BMC Developmental Biology

Figure 2

From: Ectodermal Wnt6 is an early negative regulator of limb chondrogenesis in the chicken embryo

Figure 2

Alcian-blue staining of the cartilaginous skeleton of embryonic chicken wings. A-C Control wings. A1-C1 Wings after injection of Wnt6-CHO cells at HH-stage 20-21 and 4 days reincubation. Skeletal elements are shorter in length compared to the control wings. A1 to C1 illustrate increasingly severe defects depending on the quantity and spreading of injected cells, A1 being the mildest, C1 being the severest phenotype. A1 only the humerus is affected. B1 stylopod and zeugopod are affected, C1 the entire limb is affected. Scale bar 500 μm.

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