Table 5 Primers for pyrosequencing analysis.
From: Donor-host mitochondrial compatibility improves efficiency of bovine somatic cell nuclear transfer
Genes | PCR primers(5'-3') | Length (bp) | CpG site |
|---|---|---|---|
Oct-4 | ATTTGGATGAGTTTTTAAGGGTTTT | 292 | 23 |
| Â | ACTCCAACTTCTCCTTATCCAACTT | Â | Â |
Nanog | TTTTTTAATTATAATTTGATGGGGT | 288 | 13 |
| Â | CTAACACACCTTAAATAAACAAACC | Â | Â |
Sox2 | GGGATATGATTAGTATGTATTTTTT | 230 | 15 |
| Â | TTCTCCATACTATTTCTTACTATCCTCC | Â | Â |