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Table 1 In vitro preimplantation development of bovine embryos reconstructed with adult fibroblast cells.

From: Donor-host mitochondrial compatibility improves efficiency of bovine somatic cell nuclear transfer

SCNT type

Homotype (Oocytes-Donor)

Heterotype (Oocytes-Donor)

IVF

 

A-A

B-B

A-B

B-A

/

Oocytes

497

1608

672

631

229

No. of replicates

14

40

16

15

7

Oocytes maturation (%)

392 (78.9%)a

1221 (75.9%)a

518 (77.1%)a

478 (75.8%)a

177 (77.3%)a

*KCC

335

963

426

402

/

Fused and cultured

187

626

258

267

/

Cleaved (%)f

135 (72.2)a

455 (72.7%)a

188 (72.9)a

184 (68.9)a

111

8-cell (%)g

100 (74.0)a

295 (64.8)b

118 (62.8)b

103 (56.0)c

NA

Blastocyst (%)g

63.46.7)a

188.41.3)b

61.32.4)c

46.25.0)d

45 (40.5)b

 

251/590 (42.5%)a

107/372 (28.8%)b

/

  1. *KCC, karyoplast--cytoplast complexes. Values with different superscript letters a, b, c, d differ with statistical significance (P < 0.05 by t test). f: Percentage based on number of surviving and fused reconstructed embryos, which were activated and cultured following KCC fusion. g: Percentage based on number of cleaved reconstructed embryos.