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Table 1 Peptides identified by LTQ and FTICR-MSMS in quail diencephalon.

From: Neuropeptidomic analysis of the embryonic Japanese quail diencephalon

Precursor

Peptide

MH+

Sequence

Comment

PCA & PLS/DA1

Acyl-CoA binding protein

ODN peptide

1637.56

TVGDVNTDRPGMLDF

 

Yes

Cerebellin-1 pre.

Cerebellin-1

1632.85

SGSAKVAFSAIRSTNH

 

Yes

Cerebellin-2 pre.

Cerebellin-2

1620.80

SGSAKVAFSATRSTNH

  

Cerebellin-4 pre.

Cerebellin-4

1451.70

ANSKVAFSAVRSTN

 

Yes

 

Cerebellin-4

1267.36

SKVAFSAVRSTN

 

Yes

Gastrin-releasing peptide

GRP

1766.69

APLQPGGSPALTKIYPR

 

Yes

 

GRP

1608.89

APLQPGGSPALTKIYP

  

Gonadotropin-inhibiting hormone

GnIH propep

1743.83

SVPISLSQGVQESEPGM

  
 

GnIH-RP2

1723.97

SPLARSSIQSLLNLPQ

  
 

GnIH-RP2

1426.80

ARSSIQSLLNLPQ

 

Yes

Glucagon family neuropeptides

PACAP27

1573.73

HIDGIFTDSYSRY

  

Neuropeptide Y (NPY) pre.

NPY

1443.81

SSPETLISDLLLR

  

Prepronociceptin (PNOC) pre

Precursor

1226.82

AVASPLQVSELL

 

Yes

 

Nociceptin

1981.07

YGGFIGVRKSARKWNNQ

 

Yes

 

Nociceptin

1154.63

YGGFIGVRKSA

 

Yes

 

Nociceptin

996.56

YGGFIGVRK

  
 

Neuropeptide1

1981.05

GSWPAARGVQ

 

Yes

 

Neuropeptide2

1702.86

FSEFLKQYLGMSPR

 

Yes

 

Neuropeptide2

1555.79

SEFLKQYLGMSPR

 

Yes

Proenkephalin A (PENK) pre

Precursor

2218.94

MDELYHPESEDEANGGEILA*

  
 

Precursor

2147.91

MDELYHPESEDEANGGEIL*

  
 

Precursor

2087.90

DELYHPESEDEANGGEILA*

  
 

Precursor

1972.44

ELYHPESEDEANGGEILA*

 

Yes

 

Precursor

1901.84

ELYHPESEDEANGGEIL*

  
 

Precursor

1843.83

LYHPESEDEANGGEILA*

  
 

Precursor

1730.75

YHPESEDEANGGEILA*

  
 

Precursor

1659.72

YHPESEDEANGGEIL*

  
 

Precursor

1496.65

HPESEDEANGGEIL*

  
 

Precursor

1448.69

VGRPEWWLDYQ**

  
 

Precursor

1387.62

SPELEDEAKELQ*

 

Yes

 

Precursor

1259.90

SPELEDEAKEL*

 

Yes

 

Precursor

1203.57

ELEDEAKELQ*

  
 

Precursor

1074.99

LEDEAKELQ*

 

Yes

 

MERF

877.40

YGGFMRF

 

Yes

 

MERSL

930.45

YGGFMRSL

 

Yes

Proenkephalin B (PENK) pre

Precursor

1028.80

PKLKWDNQ

 

Yes

Protachykinin 1 (PPT) pre

C-term flanking peptide (CTFP)

1741.07

SLNSGSSERSIAQNYE

 

Yes

 

C-term flanking peptide (CTFP)

1821.77

SLNSGSSERSIAQNYE

S6/7(p)

Yes

 

C-term flanking peptide (CTFP)

1900.26

SLNSGSSERSIAQNYE

S6/7, S10(p)

Yes

 

Substance P

   

Yes

 

Neuropeptide K

947.42

DAGYGQISH

 

Yes

 

Neuropeptide K

832.40

AGYGQISH

  
 

Neurokinin A

1133.87

HKTDSFVGLM

M-amide

Yes

Secretogranin-1 pre

Secretogranin1- propep

1725.81

QYDKMDQLAQLLNY

Pyroglutamic Acid

 
 

Secretogranin1

1357.54

IHEGEEGEAEEE

 

Yes

Secretogranin-2 pre

Secretoneurin

2064.99

TNEIVEEQYTPQSLATLE

  
 

Secretoneurin

1650.79

TNEIVEEQYTPQSL

 

Yes

 

Secretoneurin

1537.70

TNEIVEEQYTPQS

  
 

Secretogranin2

1255.59

SGKLSFLEDEM

  
 

Secretogranin2

1124.55

SGKLSFLEDE

 

Yes

 

Secretogranin2

880.478

SGKLSFLE

  

Secretogranin-5 pre

C-terminal peptide (CTP)

1772.96

SVNPYLQGKRLDNVVA

 

Yes

 

C-terminal peptide (CTP)

1701.63

SVNPYLQGKRLDNVV

 

Yes

Somatostatin pre

SMS-propep

1256.71

SLAAAAGKQELAK

  
 

SMS-14

1409.18

KNFFWKTFTSC

 

Yes

 

SMS-14

1019.90

FWKTFTSC

 

Yes

 

SMS-28

1226.61

SANSNPALAPRE

 

Yes

 

SMS-28

1097.57

SANSNPALAPR

  
 

SMS-28

1139.58

ANSNPALAPRE

  

Vasoactive intestinal peptide

VIP

1219.7

AVFTDNYSRF

 

Yes

CRMP-2

 

1210.81

APPGGRANITSLG

 

Yes

FKBP5

 

1712.77

ANAGPNTNGSQFFICTA

 

Yes

Stathmin

 

1358.74

ASGQAFELILGPR

  

Thymosin beta

 

1566.84

SDKPDMAEIEKFDK

 

Yes

  1. 1 Partial Least Square Discriminant Analysis * Propeptide to Met-enkephalin.
  2. **Propeptide to Leu-enkephalin