Figure 5From: Molecular profiling of the developing mouse axial skeleton: a role for Tgfbr2 in the development of the intervertebral discVerification of regulation of selected genes by TGF-β using RT-PCR. Sclerotome was treated with TGF-β1 for 8 hours at which time RNA was isolated, cDNA was made and semiquantitative RT-PCR performed. Beta-2-microglobulin (b2 mg) was used as a loading control. Product formation in the linear range is shown.Back to article page