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Table 1 PCR primers used for Sequenom analysis

From: DNA methylation patterns in tissues from mid-gestation bovine foetuses produced by somatic cell nuclear transfer show subtle abnormalities in nuclear reprogramming

Name

Sequence

Chromosomal location

Product size (bp)

SNRPN_L

AGGAAGAGAGTTGGGAGGTATTATTTTGGGTTGAA

ChrU:24360-23919

437

SNRPN_R

CAGTAATACGACTCACTATAGGGAGAAGGCTAACCCCAAACCTCCAAAAATTATC

  

IGF2_L

AGGAAGAGAGGGGTATTTGGGGTAGTTTAGG

Chr29:3633319-3633749

521

IGF2_R

CAGTAATACGACTCACTATAGGGAGAAGGCTATTCTAATCCCCTCAACCAAATAAA

  

CDKN1C_L

AGGAAGAGAGGTAGTGGTATATTTAGTTGGAAGTTGTAGT

Chr29:2954381-2953897

485

CDKN1C_R

CAGTAATACGACTCACTATAGGGAGAAGGCTTAGTTAGGTTAGAGTTAGTT

  

HAND1_L

AGGAAGAGAGGAGAAAGGTTTTTGGGGATAAAATT

Chr7:1101938-1101390

549

HAND1_R

CAGTAATACGACTCACTATAGGGAGAAGGCTCAAACCCTACAACTAACAAAACATCC

  

ASCL2_L

AGGAAGAGAGGTATTAGGGGGAGTTTTGGTAG

Chr29:3526331-3526685

354

ASCL2_R

CAGTAATACGACTCACTATAGGGAGAAGGCTCTAAAACCCCAAATTCACCAACTTC

  

KCNQ1_L

AGGAAGAGAGGGGTTTGGTTAAGAAGTGTTTTTTTT

Chr29:3365714-3365215

500

KCNQ1_R

CAGTAATACGACTCACTATAGGGAGAAGGCTAATCAAACCCACAAAACCCTAAACTT

  

Dio3_L

AGGAAGAGAGTTTGTATTTGTTTGGTTTGTTTTAA

Chr21:1453786-1454238

453

Dio3_R

CAGTAATACGACTCACTATAGGGAGAAGGCTCAACTCTTCATCAACAATAAAACTC

  

KCNQ1OT1_L

AGGAAGAGAGTAGTTGATTGGATAGTTTGTAGGGG

Chr29:3133697-3133352

346

KCNQ1OT1_R

CAGTAATACGACTCACTATAGGGAGAAGGCTCCACAAATATTCCTCAAAATCACTC

  

STAT5_L

AGGAAGAGAGTTTGTTAGAGGTAGTTGATTTTTGAGGA

Chr19:872990-873467

478

STAT5_R

CAGTAATACGACTCACTATAGGGAGAAGGCTAAAAAAACAAAACACTCCCTCTCTC

  

GR_R

CAGTAATACGACTCACTATAGGGAGAAGGCTAATTTTCTCTATAATTTCTCTTCTTACC

Chr7: 2747426-2747103

324

GR_L

AGGAAGAGAGTTTTTTTGAAGTTTTTTTAGAGGG

  

DKK_001_L

AGGAAGAGAGTTTTTTTTGAGTTTTTTTGAGATGA

Chr26:336954-336488

467

DKK_001_R

CAGTAATACGACTCACTATAGGGAGAAGGCTCACTTAAACACCCAATACCACACT

  

DKK_002_L

AGGAAGAGAGGTGTGGTATTGGGTGTTTAAGTGT

Chr26: 336510-336157

534

DKK_002_R

CAGTAATACGACTCACTATAGGGAGAAGGCTCCTAAAATCCTTTCTAAAAATCCTC

  

CSF-1_L

AGGAAGAGAGGTAGTTTTTGGAGTAGTTGTAGGGT

Chr3: 629601-630161

561

CSF-1_R

CAGTAATACGACTCACTATAGGGAGAAGGCTCAAAATAATTTCCCATAAACCACATAC

  

Satellite I_L

AGGAAGAGAGTGTAGATTGGGGATAGGAGAGTTAG

N/A

345

Satellite I_R

CAGTAATACGACTCACTATAGGGAGAAGGCTCCTACTTTATCTAAAAAAAATTACCTTCC

  

Satellite II_L

AGGAAGAGAGTTTGGTTTTAGGTTGGGAGTTTAAAG

N/A

278

Satellite II_R

CAGTAATACGACTCACTATAGGGAGAAGGCTAAAACAACAATCAAACACCACTCAC

  

Satellite alpha_L

AGGAAGAGAGTTTTTTTTGATTTGGATAGGAGGG

N/A

279

Satellite alpha_R

CAGTAATACGACTCACTATAGGGAGAAGGCTACTATATTTAAAACCAAAAATTTTTCC

  
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BMC Developmental Biology

ISSN: 1471-213X

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