HK, PK and G6PDH activities during A. aegypti embryogenesis. The HK (A), PK (B) and G6PDH (C) activities were measured in egg homogenates on different hours of embryo development. Each experiment was replicated three times and error bars represent standard deviation of sample means. (*p < 0.05; ***p < 0.001, ANOVA). The dashed line indicates the germ band retraction stage 24 HAE.