Aedes aegypti embryogenesis at 28°C. (A) 0 h after egg laying (HAE) embryo, detached from the endochorion. (B) 3-HAE embryo at the syncytial blastoderm stage. Insert shows the pole cells outside the blastoderm. (C) 5-HAE embryo, at the cellular blastoderm stage. Insert shows ventral-posterior region of the blastoderm detached from the endochorion and cell boundaries. (D) 10-HAE embryo in the middle of germ band extension. (E) 15-HAE embryo at the beginning of germ band retraction. (F) 24-HAE embryo at the germ band retraction stage. (G) 31-HAE embryo, at dorsal closure stage. Focal plane is located inside the embryo at the embryo-yolk junction region. (H) 48-HAE embryo at late organogenesis stage. Larvae segmentation is partially evident. (I) 62-HAE embryo at the end of embryogenesis showing the head, three fused thoracic segments, eight abdominal segments and the respiratory siphon and associated structures. In B-G, dorsal side is up. Scale bar = 100 μm. Arrow: cephalic region segmented. Arrowhead: serosal cuticle limits, detached from the endochorion. y: yolk. A-C, F, H, I: DIC microscopy. C insert: Bright field. D, E, G: Stereoscope.