Increased phosphorylated ERK1/2 and p38 and no change in phosphorylated PKCα and AKT in skull base tissues of Fgfr2+/P253Rat E17.5. A-D) Western blot analysis of different markers shows increased phosphorylated ERK1/2 and p38 and no change in phosphorylated AKT and PKCα. E, F) Scatter plots using normalized levels of of mutant phos/total/control phos/total shows increased ratio of phosphorylated ERK1/2 and p38 to total protein in the Fgfr2+/P253Ras compared to the wild-type skull bases. Each dot represents the analysis of data from one embryo. The bar represents the median value.