|
Doses of cyclophosphamide
| |
12.5 mg/kg
|
CP 20 mg/kg
|
CP 40 mg/kg
|
|---|
|
Number of tested embryos
| |
80
|
92
|
61
|
|
Type of anomalies
| |
Fetuses with malformed limbs§
|
| |
Forelimbs
|
6 (7.5%)
|
24 (26.1%)
|
59 (96.7%)
|
| | |
17.7 ± 3.5a
|
32.4 ± 3.1b
|
73.0 ± 0.5 c
|
|
Digit anomalies1 arcsine, mean ± SE
| | | | |
| |
Hindlimbs
|
23(28.8%)
|
66 (71.7%)
|
61 (100%)
|
| | |
32.5 ± 3.5a*
|
55.9 ± 3.8b*
|
78.9 ± 0.3c
|
| |
Forelimbs
|
0
|
0
|
0
|
| | |
9.9 ± 0.3
|
10.7 ± 0.4
|
11.0 ± 0.3
|
|
Limb reduction anomalies, arcsine, mean ± SE
| | | | |
| |
Hindlimbs
|
0
|
24 (26.1%)
|
61 (100%)
|
| | |
9.9 ± 0.3a
|
32.4 ± 3.1b*
|
78.9 ± 0.3c*
|
- 1Digit anomalies that were registered included syndactyly, ectrodactyly, adactyly. Limb reduction anomalies included amelia, apodia and hemimelia.
- §No fetuses with digit and limb anomalies were found in litters of control mice. Superscripts denote results of statistical analysis of values within a row. Values not sharing common superscripts are significantly different (the GT-2 test, p < 0.05).
- *Differences between the fore- and hindlimbs are statistically significant (Student's t-test, p < 0.05).
