Skip to main content

Table 2 Limb phenotypes exhibited by embryos of p53+/- mice treated with CP

From: Teratogen-induced alterations in microRNA-34, microRNA-125b and microRNA-155 expression: correlation with embryonic p53 genotype and limb phenotype

Doses of cyclophosphamide   12.5 mg/kg CP 20 mg/kg CP 40 mg/kg
Number of tested embryos   80 92 61
Type of anomalies   Fetuses with malformed limbs§
  Forelimbs 6 (7.5%) 24 (26.1%) 59 (96.7%)
   17.7 ± 3.5a 32.4 ± 3.1b 73.0 ± 0.5 c
Digit anomalies1 arcsine, mean ± SE     
  Hindlimbs 23(28.8%) 66 (71.7%) 61 (100%)
   32.5 ± 3.5a* 55.9 ± 3.8b* 78.9 ± 0.3c
  Forelimbs 0 0 0
   9.9 ± 0.3 10.7 ± 0.4 11.0 ± 0.3
Limb reduction anomalies, arcsine, mean ± SE     
  Hindlimbs 0 24 (26.1%) 61 (100%)
   9.9 ± 0.3a 32.4 ± 3.1b* 78.9 ± 0.3c*
  1. 1Digit anomalies that were registered included syndactyly, ectrodactyly, adactyly. Limb reduction anomalies included amelia, apodia and hemimelia.
  2. §No fetuses with digit and limb anomalies were found in litters of control mice. Superscripts denote results of statistical analysis of values within a row. Values not sharing common superscripts are significantly different (the GT-2 test, p < 0.05).
  3. *Differences between the fore- and hindlimbs are statistically significant (Student's t-test, p < 0.05).