Figure 1

Expression of miRNA-34, miRNA-125b and miRNA-155 in the forelimbs (FL) and hindlimbs (HL) of p53+/+ and p53+/+ embryos of mice exposed to different doses of CP. Samples were run in triplicate. Relative levels of the miRNAs were calculated using the 2-ΔΔCT method (U6B was used as an endogenous control). Results were analyzed statistically using the GT2 test for multiple comparisons (n = 4, k = 12) and presented as95% comparison intervals for the means. Means with intervals that do not overlap are significantly different. Means with intervals, which do not reach 1 (the level of expression in controls), differ significantly from controls.