Figure 6

Oocyte loss was associated with ovary disorganization following re-aggregation. (A, B) Immunofluorescence revealed TRA98+ oocytes and FOXL2+ granulosa cells in ovarian cord-like structures (dashed lines) primarily sequestered from PECAM+ endothelial cells (A) and Laminin+ basment membrane (B) following five days of intact e12.5 female genital ridge transplantation. Along with disrupted ovarian cord formation, re-aggregation also resulted in a uniform distribution of PECAM+ and Laminin+ cells amongst dispersed oocytes and granulosa cells that correlated with subsequent oocyte loss. 100× magnification.