Figure 3

Genital ridge re-aggregation did not affect oocyte meiotic entry or progression but disrupted oocyte survival and development in follicles. (A) Intact or re-aggregated (re-agg) e12.5 female genital ridges were transplanted for three days, and germ cells were subsequently analyzed for entry and progression through stages of meiosis prophase I by SYCP3 and SYCP1 immunostaining of chromosome synapsis. Blue is DAPI. 630× magnification. (B) Intact or re-aggregated e12.5 samples on day three of transplantation were quantified for the percentage of cells in meiotic prophase I as determined by SYCP chromosomal alignment localization by immunofluorescence. Error bars represent s.d. (n = 3, 100 cells/graft). No statistically significant difference was detected. (C) By 12 days of transplantation, many primordial and primary follicles were observed from the intact e12.5 genital ridge but none were detected from the re-aggregated genital ridge by H&E stain. 200× magnification.