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Figure 6 | BMC Developmental Biology

Figure 6

From: SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells

Figure 6

DAPT treatment rescues the pancreatic phenotype of Sel1lβgeo/βgeoembryos. The dorsal pancreatic bud of wild-type (+/+) and Sel1l mutant (-/-) embryos was isolated at E11.5 and cultured for 8 days in the presence of 0.1% DMSO (control) and 10 μM of DAPT. (A-D) Immunohistological analysis of DAPT-treated and non-treated pancreatic buds with the indicated antibodies. (E-F) Quantification of insulin+ cells in DMSO and DAPT-treated pancreatic explants. DAPT did not significantly change the number of insulin+ cells in wild-type pancreatic buds (A-B and E), however, it induced a marked increase of insulin+ cells in Sel1l mutant pancreatic buds (C-D and F). Scale bar: 100 μm.

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