Figure 5
From: SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells
Sel1l -deficient pancreatic epithelial cells fail to commit to lineage precursors during the secondary transition. (A-D) Immunohistological analysis of the developing dorsal pancreas of wild-type and Sel1lβgeo/βgeoembryos at E15, using the indicated antibodies. The genotypes of the pancreatic sections are indicated as "+/+" and "-/-", respectively. (A-B) PTF1a is expressed in the periphery of wild-type pancreatic epithelium marking the commitment of pancreatic epithelial cells into the exocrine lineage (A, white arrows); PTF1a expression is absent in the mutant pancreas (B). Up-regulation of PDX1 expression in a subset of epithelial cells in the core of wild-type pancreatic epithelium marks the commitment of pancreatic epithelial cells into the endocrine lineage (A, red arrows); whereas PDX1 is uniformly expressed in mutant pancreatic epithelial cells (B). (C-D) SOX9 is expressed in a small population of epithelial cells in wild-type pancreatic epithelium (C, red arrows); in contrast, SOX9 mostly co-localizes with PDX1 in mutant pancreatic epithelium (D, asterisks). Scale bar: 100 μm.