Figure 4
From: SEL1L deficiency impairs growth and differentiation of pancreatic epithelial cells
Sel1l -deficient pancreatic epithelium exhibits impaired growth, branching morphology and differentiation ex vivo. Dorsal pancreatic buds of wild-type and Sel1lβgeo/βgeoembryos were isolated at E11.5 and cultured as described in Materials and Methods. The genotypes of the pancreatic explants are indicated as "+/+" and "-/-", respectively. Cultured pancreatic explants were immunostained using the indicated antibodies. Sel1l-deficient pancreatic epithelium grows poorly and displays impaired branching morphology (B and D), as compared to wild-type control explants (A and C). The number of insulin+ cells in mutant pancreatic epithelia were markedly reduced (B, arrows), as compared to the wild-type controls (A, arrows). Amylase expression was detected in Sel1l-deficient pancreatic epithelium (D, arrows), but the expression is significantly lower than that in wild-type epithelium (C, arrows). Scale bar: 100 μm.